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ace2 expression lentiviral vector  (Addgene inc)


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    Addgene inc ace2 expression lentiviral vector
    Ace2 Expression Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ace2+expression+lentiviral+vector/RRL%2Esin%2EcPPT%2ESFFV%2FAce2%2EIRES-puro%2EWPRE+(MT126)+(Plasmid+%23145839)/pm40562869-291-15-19
    Average 93 stars, based on 23 article reviews
    ace2 expression lentiviral vector - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models
    Article Snippet: Ultimately, an ImmunoScan Entry analyser (Cellular Technology, Shaker Heights, USA) was used to count the spots. .. Transfected 293 T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models.
    Article Snippet: Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). .. Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Expressing:

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models
    Article Snippet: Ultimately, an ImmunoScan Entry analyser (Cellular Technology, Shaker Heights, USA) was used to count the spots. .. Transfected 293 T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models.
    Article Snippet: Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). .. Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Article Title: Spatiotemporal Dynamic Immunomodulation by Infection‐Mimicking Gels Enhances Broad and Durable Protective Immunity Against Heterologous Viruses
    Article Snippet: Sixty hours post transfection, SARS‐CoV‐2 spike pseudoviruses containing culture supernatants were harvested, filtered (0.45 μm pore size, Millipore); the copy number of pseudoviruses was quantitated by qPCR (Takara Bio). .. Derivative 293T cells expressing angiotensin converting enzyme 2 (ACE2) were generated by transfecting 293T cells with ACE2 expression lentiviral vector (Addgene Plasmid, 145839). ..

    Article Title: Spatiotemporal Dynamic Immunomodulation by Infection-Mimicking Gels Enhances Broad and Durable Protective Immunity Against Heterologous Viruses.
    Article Snippet: Sixty hours post transfection, SARSCoV-2 spike pseudoviruses containing culture supernatants were harvested, filtered (0.45 μm pore size, Millipore); the copy number of pseudoviruses was quantitated by qPCR (Takara Bio). .. Pseudovirus Neutralization Assay: Derivative 293T cells expressing angiotensin converting enzyme 2 (ACE2) were generated by transfecting 293T cells with ACE2 expression lentiviral vector (Addgene Plasmid, 145839). ..

    Article Title: Multiscale dynamic immunomodulation by a nanoemulsified Trojan-TLR7/8 adjuvant for robust protection against heterologous pandemic and endemic viruses
    Article Snippet: .. Derivatives of 293T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, 145839). ..

    Article Title: Multiscale dynamic immunomodulation by a nanoemulsified Trojan-TLR7/8 adjuvant for robust protection against heterologous pandemic and endemic viruses.
    Article Snippet: .. Derivatives of 293T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, 145839). ..

    Generated:

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models
    Article Snippet: Ultimately, an ImmunoScan Entry analyser (Cellular Technology, Shaker Heights, USA) was used to count the spots. .. Transfected 293 T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models.
    Article Snippet: Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). .. Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Article Title: Spatiotemporal Dynamic Immunomodulation by Infection‐Mimicking Gels Enhances Broad and Durable Protective Immunity Against Heterologous Viruses
    Article Snippet: Sixty hours post transfection, SARS‐CoV‐2 spike pseudoviruses containing culture supernatants were harvested, filtered (0.45 μm pore size, Millipore); the copy number of pseudoviruses was quantitated by qPCR (Takara Bio). .. Derivative 293T cells expressing angiotensin converting enzyme 2 (ACE2) were generated by transfecting 293T cells with ACE2 expression lentiviral vector (Addgene Plasmid, 145839). ..

    Article Title: Spatiotemporal Dynamic Immunomodulation by Infection-Mimicking Gels Enhances Broad and Durable Protective Immunity Against Heterologous Viruses.
    Article Snippet: Sixty hours post transfection, SARSCoV-2 spike pseudoviruses containing culture supernatants were harvested, filtered (0.45 μm pore size, Millipore); the copy number of pseudoviruses was quantitated by qPCR (Takara Bio). .. Pseudovirus Neutralization Assay: Derivative 293T cells expressing angiotensin converting enzyme 2 (ACE2) were generated by transfecting 293T cells with ACE2 expression lentiviral vector (Addgene Plasmid, 145839). ..

    Article Title: Multiscale dynamic immunomodulation by a nanoemulsified Trojan-TLR7/8 adjuvant for robust protection against heterologous pandemic and endemic viruses
    Article Snippet: .. Derivatives of 293T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, 145839). ..

    Article Title: Multiscale dynamic immunomodulation by a nanoemulsified Trojan-TLR7/8 adjuvant for robust protection against heterologous pandemic and endemic viruses.
    Article Snippet: .. Derivatives of 293T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, 145839). ..

    Plasmid Preparation:

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models
    Article Snippet: Ultimately, an ImmunoScan Entry analyser (Cellular Technology, Shaker Heights, USA) was used to count the spots. .. Transfected 293 T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models.
    Article Snippet: Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). .. Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Article Title: Spatiotemporal Dynamic Immunomodulation by Infection‐Mimicking Gels Enhances Broad and Durable Protective Immunity Against Heterologous Viruses
    Article Snippet: Sixty hours post transfection, SARS‐CoV‐2 spike pseudoviruses containing culture supernatants were harvested, filtered (0.45 μm pore size, Millipore); the copy number of pseudoviruses was quantitated by qPCR (Takara Bio). .. Derivative 293T cells expressing angiotensin converting enzyme 2 (ACE2) were generated by transfecting 293T cells with ACE2 expression lentiviral vector (Addgene Plasmid, 145839). ..

    Article Title: Spatiotemporal Dynamic Immunomodulation by Infection-Mimicking Gels Enhances Broad and Durable Protective Immunity Against Heterologous Viruses.
    Article Snippet: Sixty hours post transfection, SARSCoV-2 spike pseudoviruses containing culture supernatants were harvested, filtered (0.45 μm pore size, Millipore); the copy number of pseudoviruses was quantitated by qPCR (Takara Bio). .. Pseudovirus Neutralization Assay: Derivative 293T cells expressing angiotensin converting enzyme 2 (ACE2) were generated by transfecting 293T cells with ACE2 expression lentiviral vector (Addgene Plasmid, 145839). ..

    Article Title: Multiscale dynamic immunomodulation by a nanoemulsified Trojan-TLR7/8 adjuvant for robust protection against heterologous pandemic and endemic viruses
    Article Snippet: .. Derivatives of 293T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, 145839). ..

    Article Title: Multiscale dynamic immunomodulation by a nanoemulsified Trojan-TLR7/8 adjuvant for robust protection against heterologous pandemic and endemic viruses.
    Article Snippet: .. Derivatives of 293T cells expressing ACE2 were generated by transducing 293 T cells with an ACE2 expression lentiviral vector (Addgene plasmid, 145839). ..

    Neutralization:

    Article Title: Kinetically activating nanovaccine mimicking multidimensional immunomodulation of natural infection for broad protection against heterologous viruses in animal models.
    Article Snippet: Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). .. Serum neutralization against SARS-CoV-2 pseudovirus (pseudotyped SARS-CoV-2) Transfected 293 T cells expressing ACE2 were generated by transducing 293T cells with an ACE2 expression lentiviral vector (Addgene plasmid, cat. no. 145839). ..

    Article Title: Spatiotemporal Dynamic Immunomodulation by Infection-Mimicking Gels Enhances Broad and Durable Protective Immunity Against Heterologous Viruses.
    Article Snippet: Sixty hours post transfection, SARSCoV-2 spike pseudoviruses containing culture supernatants were harvested, filtered (0.45 μm pore size, Millipore); the copy number of pseudoviruses was quantitated by qPCR (Takara Bio). .. Pseudovirus Neutralization Assay: Derivative 293T cells expressing angiotensin converting enzyme 2 (ACE2) were generated by transfecting 293T cells with ACE2 expression lentiviral vector (Addgene Plasmid, 145839). ..



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    Addgene inc ace2 expression lentiviral vector
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    ( A ) Immunoblot analysis of lysates prepared from <t>MRC-5-ACE2</t> human cells that were mock-infected or infected with SARS-CoV-2 at multiplicity of infection (MOI) of 5 for 24 or 48 hr. The immunoblot was probed with anti-TRMT1, actin, or SARS-CoV-2 nucleocapsid (N) antibodies. Circle represents endogenous full-length TRMT1. Asterisk (*) denotes a non-specific band. Size markers are noted in kiloDalton. ( B ) Quantification of TRMT1 signal intensity normalized to actin in the mock or SARS-CoV-2-infected cell lines. TRMT1 protein levels are expressed relative to mock-infected samples for each time point. ( C ) m2,2G levels in small RNAs isolated from MRC5 cells that were either mock-infected or infected with SARS-CoV-2 at MOI of 5 for 24 or 48 hr. m2,2G levels were normalized to A, C, G, and U. Samples were measured in biological replicates. Statistical significance for ( B ) and ( C ) was determined by two-way ANOVA with multiple comparisons test. ***p<0.001; ****p<0.0001; ns, non-significant. ( D ) Levels of the indicated RNA modifications in small RNAs isolated from MRC5 cells that were either mock-infected or infected with SARS-CoV-2 for 24 or 48 hr. RNA modification levels were normalized to A, C, G, and U. Y-axis represents the log2 fold change in the levels of the indicated tRNA modification between SARS-CoV-2 infected versus mock-infected MRC5 cells. The experiment in ( C ) was repeated as an independent biological replicate in . Figure 1—source data 1. Raw uncropped immunoblots for . Figure 1—source data 2. LC-MS measurements of RNA modifications.
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    ( A ) Immunoblot analysis of lysates prepared from <t>MRC-5-ACE2</t> human cells that were mock-infected or infected with SARS-CoV-2 at multiplicity of infection (MOI) of 5 for 24 or 48 hr. The immunoblot was probed with anti-TRMT1, actin, or SARS-CoV-2 nucleocapsid (N) antibodies. Circle represents endogenous full-length TRMT1. Asterisk (*) denotes a non-specific band. Size markers are noted in kiloDalton. ( B ) Quantification of TRMT1 signal intensity normalized to actin in the mock or SARS-CoV-2-infected cell lines. TRMT1 protein levels are expressed relative to mock-infected samples for each time point. ( C ) m2,2G levels in small RNAs isolated from MRC5 cells that were either mock-infected or infected with SARS-CoV-2 at MOI of 5 for 24 or 48 hr. m2,2G levels were normalized to A, C, G, and U. Samples were measured in biological replicates. Statistical significance for ( B ) and ( C ) was determined by two-way ANOVA with multiple comparisons test. ***p<0.001; ****p<0.0001; ns, non-significant. ( D ) Levels of the indicated RNA modifications in small RNAs isolated from MRC5 cells that were either mock-infected or infected with SARS-CoV-2 for 24 or 48 hr. RNA modification levels were normalized to A, C, G, and U. Y-axis represents the log2 fold change in the levels of the indicated tRNA modification between SARS-CoV-2 infected versus mock-infected MRC5 cells. The experiment in ( C ) was repeated as an independent biological replicate in . Figure 1—source data 1. Raw uncropped immunoblots for . Figure 1—source data 2. LC-MS measurements of RNA modifications.
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    Image Search Results


    ( A ) Immunoblot analysis of lysates prepared from MRC-5-ACE2 human cells that were mock-infected or infected with SARS-CoV-2 at multiplicity of infection (MOI) of 5 for 24 or 48 hr. The immunoblot was probed with anti-TRMT1, actin, or SARS-CoV-2 nucleocapsid (N) antibodies. Circle represents endogenous full-length TRMT1. Asterisk (*) denotes a non-specific band. Size markers are noted in kiloDalton. ( B ) Quantification of TRMT1 signal intensity normalized to actin in the mock or SARS-CoV-2-infected cell lines. TRMT1 protein levels are expressed relative to mock-infected samples for each time point. ( C ) m2,2G levels in small RNAs isolated from MRC5 cells that were either mock-infected or infected with SARS-CoV-2 at MOI of 5 for 24 or 48 hr. m2,2G levels were normalized to A, C, G, and U. Samples were measured in biological replicates. Statistical significance for ( B ) and ( C ) was determined by two-way ANOVA with multiple comparisons test. ***p<0.001; ****p<0.0001; ns, non-significant. ( D ) Levels of the indicated RNA modifications in small RNAs isolated from MRC5 cells that were either mock-infected or infected with SARS-CoV-2 for 24 or 48 hr. RNA modification levels were normalized to A, C, G, and U. Y-axis represents the log2 fold change in the levels of the indicated tRNA modification between SARS-CoV-2 infected versus mock-infected MRC5 cells. The experiment in ( C ) was repeated as an independent biological replicate in . Figure 1—source data 1. Raw uncropped immunoblots for . Figure 1—source data 2. LC-MS measurements of RNA modifications.

    Journal: eLife

    Article Title: Proteolytic cleavage and inactivation of the TRMT1 tRNA modification enzyme by SARS-CoV-2 main protease

    doi: 10.7554/eLife.90316

    Figure Lengend Snippet: ( A ) Immunoblot analysis of lysates prepared from MRC-5-ACE2 human cells that were mock-infected or infected with SARS-CoV-2 at multiplicity of infection (MOI) of 5 for 24 or 48 hr. The immunoblot was probed with anti-TRMT1, actin, or SARS-CoV-2 nucleocapsid (N) antibodies. Circle represents endogenous full-length TRMT1. Asterisk (*) denotes a non-specific band. Size markers are noted in kiloDalton. ( B ) Quantification of TRMT1 signal intensity normalized to actin in the mock or SARS-CoV-2-infected cell lines. TRMT1 protein levels are expressed relative to mock-infected samples for each time point. ( C ) m2,2G levels in small RNAs isolated from MRC5 cells that were either mock-infected or infected with SARS-CoV-2 at MOI of 5 for 24 or 48 hr. m2,2G levels were normalized to A, C, G, and U. Samples were measured in biological replicates. Statistical significance for ( B ) and ( C ) was determined by two-way ANOVA with multiple comparisons test. ***p<0.001; ****p<0.0001; ns, non-significant. ( D ) Levels of the indicated RNA modifications in small RNAs isolated from MRC5 cells that were either mock-infected or infected with SARS-CoV-2 for 24 or 48 hr. RNA modification levels were normalized to A, C, G, and U. Y-axis represents the log2 fold change in the levels of the indicated tRNA modification between SARS-CoV-2 infected versus mock-infected MRC5 cells. The experiment in ( C ) was repeated as an independent biological replicate in . Figure 1—source data 1. Raw uncropped immunoblots for . Figure 1—source data 2. LC-MS measurements of RNA modifications.

    Article Snippet: For infections, the cells were previously transduced with a Lentiviral vector expressing ACE2 using the lentiviral construct RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136) was kindly provided by Caroline Goujon (Addgene plasmid # 145842) ( ).

    Techniques: Western Blot, Infection, Isolation, RNA modification, Modification, Liquid Chromatography with Mass Spectroscopy

    ( A ) Immunoblot analysis of lysates prepared from the indicated 293T cell lines expressing empty vector or ACE2. The immunoblot was probed with anti-ACE2 and actin. ( B ) Immunoblot analysis of lysates prepared from 293T-ACE2 cell lines that were mock-infected (multiplicity of infection, MOI 0) or infected with SARS-CoV-2 at the indicated MOI. The blot was probed against the SARS-CoV-2 nucleocapsid ( N ) and actin. Figure 6—figure supplement 1—source data 1. Raw uncropped immunoblots for .

    Journal: eLife

    Article Title: Proteolytic cleavage and inactivation of the TRMT1 tRNA modification enzyme by SARS-CoV-2 main protease

    doi: 10.7554/eLife.90316

    Figure Lengend Snippet: ( A ) Immunoblot analysis of lysates prepared from the indicated 293T cell lines expressing empty vector or ACE2. The immunoblot was probed with anti-ACE2 and actin. ( B ) Immunoblot analysis of lysates prepared from 293T-ACE2 cell lines that were mock-infected (multiplicity of infection, MOI 0) or infected with SARS-CoV-2 at the indicated MOI. The blot was probed against the SARS-CoV-2 nucleocapsid ( N ) and actin. Figure 6—figure supplement 1—source data 1. Raw uncropped immunoblots for .

    Article Snippet: For infections, the cells were previously transduced with a Lentiviral vector expressing ACE2 using the lentiviral construct RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136) was kindly provided by Caroline Goujon (Addgene plasmid # 145842) ( ).

    Techniques: Western Blot, Expressing, Plasmid Preparation, Infection

    Immunoblot of lysates from human cells integrated with empty lentiviral vector or lentiviral expression vectors for wild-type (WT) TRMT1 or TRMT1-Q530N. The cell lines were transfected with either a vector or a construct expressing Nsp5-Strep. The immunoblot was probed with anti-TRMT1, Strep, and actin antibodies. Square represents TRMT1-FLAG, circle represent endogenous TRMT1, Asterisk (*) denotes a non-specific band, and arrow represents N-terminal TRMT1 cleavage product. Figure 7—figure supplement 1—source data 1. Raw uncropped immunoblots for .

    Journal: eLife

    Article Title: Proteolytic cleavage and inactivation of the TRMT1 tRNA modification enzyme by SARS-CoV-2 main protease

    doi: 10.7554/eLife.90316

    Figure Lengend Snippet: Immunoblot of lysates from human cells integrated with empty lentiviral vector or lentiviral expression vectors for wild-type (WT) TRMT1 or TRMT1-Q530N. The cell lines were transfected with either a vector or a construct expressing Nsp5-Strep. The immunoblot was probed with anti-TRMT1, Strep, and actin antibodies. Square represents TRMT1-FLAG, circle represent endogenous TRMT1, Asterisk (*) denotes a non-specific band, and arrow represents N-terminal TRMT1 cleavage product. Figure 7—figure supplement 1—source data 1. Raw uncropped immunoblots for .

    Article Snippet: For infections, the cells were previously transduced with a Lentiviral vector expressing ACE2 using the lentiviral construct RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136) was kindly provided by Caroline Goujon (Addgene plasmid # 145842) ( ).

    Techniques: Western Blot, Plasmid Preparation, Expressing, Transfection, Construct

    The immunoblot was probed with anti-ACE2 and actin. Figure 7—figure supplement 2—source data 1. Raw uncropped immunoblots for .

    Journal: eLife

    Article Title: Proteolytic cleavage and inactivation of the TRMT1 tRNA modification enzyme by SARS-CoV-2 main protease

    doi: 10.7554/eLife.90316

    Figure Lengend Snippet: The immunoblot was probed with anti-ACE2 and actin. Figure 7—figure supplement 2—source data 1. Raw uncropped immunoblots for .

    Article Snippet: For infections, the cells were previously transduced with a Lentiviral vector expressing ACE2 using the lentiviral construct RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136) was kindly provided by Caroline Goujon (Addgene plasmid # 145842) ( ).

    Techniques: Western Blot

    Journal: eLife

    Article Title: Proteolytic cleavage and inactivation of the TRMT1 tRNA modification enzyme by SARS-CoV-2 main protease

    doi: 10.7554/eLife.90316

    Figure Lengend Snippet:

    Article Snippet: For infections, the cells were previously transduced with a Lentiviral vector expressing ACE2 using the lentiviral construct RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136) was kindly provided by Caroline Goujon (Addgene plasmid # 145842) ( ).

    Techniques: Virus, Software, Recombinant, Plasmid Preparation, Expressing, Variant Assay, Suspension, Protein Purification, Western Blot, Imaging, Sequencing